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KMID : 0545119980080010089
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Journal of Microbiology and Biotechnology
1998 Volume.8 No. 1 p.89 ~ p.91
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Inhibition of DNA Topoisomeras Ⅰby Cryptotanshinone from Salvia miltiorrhiza
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Lee Dong-Sun
Hong Soon-Duck
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Abstract
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Cryptotanshinone induced topoisomerase Ⅰ-mediated DNA cleavage in vitro as strongly as camptothecin, whereas topoisomerase Ⅱ-mediated DNA cleavage was not induced by this agent. In DNA relaxation assay using calf thymus DNA topoisomerase I and supercoiled pBR322 DNA, cryptotanshinone inhibited topoisomerase Ⅰ-mediated DNA relaxation in a dose-dependent manner. In unwinding assay, cryptotanshinone (50μM) did not shift the topoisomers of DNA. These results suggest that cryptotanshinone exerted a preferential inhibition of topoisomerase I without intercalating into DNA.
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